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| 3. |  | RAIJ, B. van; MASCARENHAS, H. A. A.; PEREIRA, J. C. V. N. A; IGUE, T.; SORDI, G. de. Efeito de calcario e de gesso para soja cultivada em latossolo roxo acido saturado com sulfato. Revista Brasileira de Ciencia do Solo, v.18, p.305-312, 1994. Trabalho apresentado no XXIV Congresso Brasileiro de Ciencia do Solo, realizado em Goiania (GO), de 25 a 31 de julho de 1993.| Biblioteca(s): Embrapa Soja. |
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| 4. | | SAWAZAKI, E.; CASTRO, J. L.; SORDI, G. de; FANTIN, G. M.; DUDIENAS, C.; LORDELLO, A. I. L.; LARDELLO, R. R. A. Características agronômicas e de fitossanidade de híbridos exóticos de milho pipoca. In: CONGRESSO NACIONAL DE MILHO E SORGO, 22., 1998, Recife. Globalização e segurança alimentar: anais. Recife: ABMS, 1998. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
03/09/2003 |
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Data da última atualização: |
03/09/2003 |
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Autoria: |
ALMEIDA, A. M. R.; ABDELNOOR, R. V.; ARIAS, C. A. A.; CARVALHO, V. P. JACOUD FILHO, D. S.; MARIN, S. R. R.; BENATO, L. C.; PINTO, M. C.; CARVALHO, C. G. P. |
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Título: |
Genotypic diversity among brazilian isolates of Macrophomina phaseolina revealed by RAPD. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Fitopatologia Brasileira, Brasília, v. 28, n. 3, p. 279-285, maio/jun. 2003. |
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Idioma: |
Português |
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Conteúdo: |
Macrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In another study the genetic similarity was evaluated among isolates from different hosts [soybean, sorghum (Sorghum bicolor), sunflower (Helianthus annuus), cowpea (Vigna unguiculata), corn (Zea mays) and wheat (Triticum aestivum)] as well as two soil samples from native areas. Results showed that more divergent isolates originated from areas with a single crop. Isolates from areas with crop rotation were less divergent, showing high similarity values and consequently formed the largest group. Amplification of the ITS region using primers ITS1 and ITS4 produced only one DNA fragment of 620 bp. None of the isolates were differentiated through PCR-RFLP. Our results demonstrated genetic variability among Brazilian isolates of M. phaseolina and showed that one single root can harbor more than one haplotype. Moreover, cultivation with crop rotation tends to induce less specialization of the pathogen isolates. Knowledge of this variation may be useful in screening soybean genotypes for resistance to charcoal rot. MenosMacrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In another study the genetic similarity was evaluated among isolates from different hosts [soybean, sorghum (Sorghum bicolor), sunflower (Helianthus annuus), cowpea (Vigna unguiculata), corn (Zea mays) and wheat (Triticum aestivum)] as well as two soil samples from native areas. Results showed that more divergent isolates originated from areas with a single crop. Isolates from areas with crop rotation were less divergent, showing high similarity values and consequently formed the largest group. Amplification of the ITS region using primers ITS1 and ITS4 produced only one DNA fragment of 620 bp. None of the isolates were differentiated through PCR-RFLP. Our results demonstrated genetic variability among Brazilian isolates of M. ... Mostrar Tudo |
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Categoria do assunto: |
-- |
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URL: |
https://www.scielo.br/pdf/fb/v28n3/a09v28n3.pdf
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Marc: |
LEADER 02422naa a2200205 a 4500
001 1460324
005 2003-09-03
008 2003 bl uuuu u00u1 u #d
100 1 $aALMEIDA, A. M. R.
245 $aGenotypic diversity among brazilian isolates of Macrophomina phaseolina revealed by RAPD.
260 $c2003
520 $aMacrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In another study the genetic similarity was evaluated among isolates from different hosts [soybean, sorghum (Sorghum bicolor), sunflower (Helianthus annuus), cowpea (Vigna unguiculata), corn (Zea mays) and wheat (Triticum aestivum)] as well as two soil samples from native areas. Results showed that more divergent isolates originated from areas with a single crop. Isolates from areas with crop rotation were less divergent, showing high similarity values and consequently formed the largest group. Amplification of the ITS region using primers ITS1 and ITS4 produced only one DNA fragment of 620 bp. None of the isolates were differentiated through PCR-RFLP. Our results demonstrated genetic variability among Brazilian isolates of M. phaseolina and showed that one single root can harbor more than one haplotype. Moreover, cultivation with crop rotation tends to induce less specialization of the pathogen isolates. Knowledge of this variation may be useful in screening soybean genotypes for resistance to charcoal rot.
700 1 $aABDELNOOR, R. V.
700 1 $aARIAS, C. A. A.
700 1 $aCARVALHO, V. P. JACOUD FILHO, D. S.
700 1 $aMARIN, S. R. R.
700 1 $aBENATO, L. C.
700 1 $aPINTO, M. C.
700 1 $aCARVALHO, C. G. P.
773 $tFitopatologia Brasileira, Brasília$gv. 28, n. 3, p. 279-285, maio/jun. 2003.
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Embrapa Soja (CNPSO) |
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